صفحه محصول - پاورپوینت Biotechnology and Genetic Engineering

پاورپوینت Biotechnology and Genetic Engineering (pptx) 36 اسلاید

دسته بندی : پاورپوینت

نوع فایل : PowerPoint (.pptx) ( قابل ویرایش و آماده پرینت )

تعداد اسلاید: 36 اسلاید

قسمتی از متن PowerPoint (.pptx) :

Biotechnology and Genetic Engineering Recombinant DNA and DNA Science Biotechnology The use of living cells to make products such as pharmaceuticals, foods, and beverages The use of organisms such as bacteria to protect the environment The use of DNA science for the production of products, diagnostics, and research Recombinant DNA The manipulation and combination of DNA from two sources Bacterial DNA + human gene for insulin Plant DNA + bacterial DNA - Agrobacterium tumefaciens Mouse DNA + human DNA = transgenic Recombination Insert a foreign gene into a host Plasmid ( for example, exogenous DNA) into the bacterial cell – transformation or transfection-organism referred to as transgenic ( eukaryote ) or recombinant( prokaryote) Goal – To produce many copies ( clones) of a particular gene Reporter gene – tags gene of interest – to identify the presence of a gene Vectors Plasmids Viruses Particles ( DNA coated bullets) Exogenous DNA Characteristics of a Vector Can replicate independently in the host cell – contains an Ori Has restriction sites in the vector- Polylinker cloning region Has a reporter gene that will announce its presence in the host cell Is a small size in comparison to the host chromosome for ease of isolation Restriction Enzymes and Vectors Cut Plasmid with restriction enzyme Cut gene of interest with restriction enzyme Splice together gene of interest and vector Tools for Recombination Restriction enzymes Recombinant DNA